Description
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, also known as NDuFA5, belongs to the complex I NDuFA5 subunit family. The human NDuFA5 gene codes for the B13 subunit of complex I of the respiratory chain, which transfers electrons from NADH to ubiquinone. The high degree of conservation of NDuFA5 extending to plants and fungi indicates its functional significance in the enzyme complex. The protein localizes to the inner mitochondrial membrane as part of the 7 component-containing, water soluble 'iron-sulfur protein' (IP) fraction of complex I, although its specific role is unknown. It is assumed to undergo post-translational removal of the initiator methionine and N-acetylation of the next amino acid. Recombinant human NDuFA5 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques